Reporter

Part:BBa_M12101:Design

Designed by: Grant Robinson   Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10   (2010-11-24)


PTet : B0034 : GFP in BioScaffold Form, a [0,5;7,2) BioScaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742


Design Notes

See documentation about BioScaffold restriction sites- specifically FalI and PsrI- for more information about the requirements for BioScaffold designs.



Source

This is a modified version of the BBa_I13522 part and contains a truncated terminator primarily for ease of use with BioScaffold-oriented priming and assembly.


References